Wee1 inhibitor sensitizes osteosarcoma to radiotherapy treatment

However, many patients still succumb to the disease as a result of tumor metastasis and relapse 34. WEE1 kinase regulates the onset of mitosis and is expressed in human OS. BMC Cancer. WEE1 kinase plays a dominant role in the sensitivity of cancer cells to DNA damage by inhibitory phosphorylation of Cyclin-Dependent-Kinase 1 CDC2thereby preventing mitotic entry, which is illustrated in Figure 1A[ 16 — 2027 — 33 ]. The combination of SFN and radiation treatment produced significantly greater antitumor effects on the LM8 osteosarcoma cells than either treatment alone Fig.

WEE1 inhibition sensitizes osteosarcoma to radiotherapy BMC Cancer Full Text

WEE1 inhibition sensitizes osteosarcoma to radiotherapy.

WEE1 inhibition sensitizes osteosarcoma to radiotherapy. OS patients currently treated with radiotherapy generally are inoperable, have.

The use of radiotherapy in osteosarcoma (OS) is controversial due to its radioresistance. OS patients currently treated with radiotherapy. BackgroundThe use of radiotherapy in osteosarcoma (OS) is controversial due to its radioresistance.

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OS patients currently treated with radiotherapy generally.
Combination treatment resulted in a higher percentage of cells being in sub-G1 than did SFN alone. OS patients currently treated with radiotherapy generally are inoperable, have painful skeletal metastases, refuse surgery or have undergone an intralesional resection of the primary tumor.

Curr Biol.

Clin Chim Acta. Cells were plated and treated with 4 Gy irradiation, 0. This indicates a forced progression through the G 2 cell cycle checkpoint into mitosis.

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Sawai, Y.

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Although still considered an advanced technique, the use of proton radiotherapy can be even more exactly localized to deliver a higher irradiation dose in the tumor while sparing adjacent healthy tissues. Strahlenther Onkol. PubMed Google Scholar Combined effect of SFN and radiation on cellular apoptosis. Clin Cancer Res. WEE1 inhibition sensitizes osteosarcoma to radiotherapy BMC Cancer Full Text J Clin Oncol. Although radiation alone had no effect on the cell cycle, the combination of SFN and irradiation significantly increased the number of cells in sub-G 1.

CDK4/6 Inhibitors Sensitize Rb-positive Sarcoma Cells to Wee1 Kinase current standard of care which usually involves surgery, radiation, and chemotherapy.

We show that Rb-positive cell lines treated with the CDK4/6 inhibitor . We also used U2OS (osteosarcoma) as another Rb-positive cell line in. 6,6% after radiation therapy2,3.

Wee1 inhibition has been shown to sensitize osteosarcoma cells in vitro to chemotherapy or radiation at clinically feasible is active in GCTB cell lines and is inhibited by a specific Wee1 inhibitor, MK MK‑, a selective inhibitor of Wee1 has recently been identified to be able to induce MK‑ treatment induced apoptosis of ALL cells, accompanied by.

GJ: WEE1 inhibition sensitizes osteosarcoma to radiotherapy.
GK supervised the project and critically reviewed the contents of the manuscript. This suggests that WEE1 inhibition may be a promising strategy to enhance the radiotherapy effect in patients with OS. Cancer Biol Ther. Technical progression in the field of radiotherapy has facilitated a more precise localised delivery of radiation and thus warranted dose-intensification at the site of the tumor.

Our results support this; in irradiated cells, we observed only few remaining foci of DNA damage after 24h, whereas cells treated with irradiation and WEE1-inhibitor had many residual foci after 24h, indicating that they were unable to perform DNA repair.

WEE1 inhibition sensitizes osteosarcoma to radiotherapy.

At 4 days OS and 9 days osteoblasts after treatment cell viability was assessed using the CellTiter-Blue Cell Viability Assay Promega according to the manufacturer's instructions.

Wee1 inhibitor sensitizes osteosarcoma to radiotherapy treatment

Conclusion We show that PD, a small molecule WEE1 kinase inhibitor, can abrogate the G 2 checkpoint in OS cells, pushing them into mitotic catastrophe and thus sensitizing OS cells to irradiation-induced cell death. Conventional chemotherapy has been shown to enhance the effect of radiotherapy in OS. Cancer Treat Res. Technical progression in the field of radiotherapy has facilitated a more precise localised delivery of radiation and thus warranted dose-intensification at the site of the tumor. Copy and paste a formatted citation. Int J Cancer.